Abstract
Acute Respiratory Distress Syndrome (ARDS) is a devastating cause of hypoxic respiratory failure, which continues to have high mortality. It is expected that a comprehensive systems- level approach will identify global and complex changes that contribute to the development of ARDS and subsequent repair of the damaged lung. In the last decade, powerful genome-wide analytical and informatics tools have been developed, that have provided valuable insights into the mechanisms of complex diseases such as ARDS. These tools include the rapid and precise measure of gene expression at the proteomic level. This article reviews the contemporary proteomics platforms that are available for comprehensive studies in ARDS. The challenges of various biofluids that could be investigated and some of the studies performed are also discussed.Electronic supplementary materialThe online version of this article (doi:10.1186/s40169-014-0034-1) contains supplementary material, which is available to authorized users.
Highlights
Biological systems function via intricate orchestrated cellular processes in which various cellular entities participate in a tightly regulated manner
Some of these reviews have described the principles of electrophoresis, the gel based methodologies and basic principles of mass spectrometry (MS) [7]
Separation methods Prior to MS protein identification and measurement peptide mixtures such as a protein from excised gel band could be separated by I-D liquid chromatography (LC) [39]. 2D- LC is used for fractionation of complex peptide mixtures such as tissue or cellular proteins [29]
Summary
Biological systems function via intricate orchestrated cellular processes in which various cellular entities participate in a tightly regulated manner. ‘Bottom-up’ proteomics studies are typically implemented for discovery-based experiments that provide protein identification and can provide relative and absolute protein quantitative measurements with the appropriate experimental design.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
