Abstract
The objective of this study was to detect Mycoplasma bovis specific antibodies using a recently reported MilA ELISA with the aim to detect M. bovis antibodies in milk. An indirect ELISA, based on a recombinant fragment of the Mycoplasma immunogenic lipase A (MilA) protein, was conducted on 291 milk samples for the detection of M. bovis antibodies. Samples were also tested with conventional Mycoplasma culture and M. bovis PCR. Samples were collected from individual cows from 2 commercial dairy herds in South Australia. Of 291 samples tested, 68 (23.4%) were detected positive for M. bovis antibodies, 150 (51.5%) were positive for M. bovis in PCR and 166 (57.0%) in bacterial culture. These results indicate that MilA indirect ELISA can be utilized for the detection of M. bovis antibodies in milk.
Highlights
The difficulties in detection of M. bovis from milk samples via culture or Polymerase Chain Reaction (PCR) is commonly attributed to the intermittent shedding of the pathogen in milk [6], or the inability of current diagnostic
Other commercial ELISA kits have been used for M. bovis antibodies screening at bulk tank milk levels in Danish and Australian dairy herds [5,9,10]
Results of the ELISA indicated that 68/291 samples (23.4%) were positive for M. bovis antibodies (≥140 antibody units (AU))
Summary
The difficulties in detection of M. bovis from milk samples via culture or Polymerase Chain Reaction (PCR) is commonly attributed to the intermittent shedding of the pathogen in milk [6], or the inability of current diagnostic. Identifying a diagnostic test capable of accurately detecting M. bovis in milk samples from carrier cows is necessary. Bovis antibodies with the MilA ELISAs in milk samples and compare these results to the presence or absence of A total of 291 milk samples were collected aseptically from individual cow once from 2 commercial dairy farms in South Australia.
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