Abstract
This study assessed a technique for the isolation and differentiation of dog erythroid progenitors. Mononuclear cells from peripheral blood were cultured in a two-phase liquid culture system. The colony burst was confirmed after the first-phase culture by using a semi-solid culture containing methylcellulose. By immunofluorescent observation or reverse transcriptase-polymerase chain reaction analysis, the cells which proliferated during the second-phase culture were found to express glycophorin, globin, and glutamate/aspartate transporter, which are markers of dog erythrocytes. This system is beneficial for the analysis of dog erythrocytes as a reasonable amount of well-matured cells (0.5×106/ml) are obtainable.
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