Abstract
Multiple sclerosis (MS) is a serious, one of the most common disorders of the nervous system and is still uncharted course and etiology. Modern medicine characterized MS as a disease of multifactorial, complex pathogenesis. The studies were designed to test some new dependencies and determine exposure to selected environmental factors, including passive and active smoking using separation techniques in biological materials sampled noninvasively. The aim of this research was to determine by high-performance thin-layer chromatography (HPTLC) with densito metry major nicotine metabolites in urine samples taken from people with multiple sclerosis in earlier described conditions, but using new version of sandwich chamber, allowing to achieve better separation results. Urine samples (28) were collected noninvasively from patients with multiple sclerosis living in the region of Upper Silesia, Poland. The control samples (21) came from healthy people — family and friends of people with MS. Including active and pa...
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