Abstract
Persiscaria tenulla, commonly known as Polygonum, is a plant belonging to the family Polygonaceae, which originated from and is widely found in Southeast Asia countries, such as Indonesia, Malaysia, Thailand, and Vietnam. The leaf of the plant is believed to have active ingredients that are responsible for therapeutic effects. In order to take full advantage of a natural medicinal plant for the application in the pharmaceutical and food industries, extraction and separation techniques are essential. In this study, an emerging and rapid extraction approach known as liquid biphasic flotation (LBF) is proposed for the extraction of protein from Persiscaria tenulla leaves. The scope of this study is to establish an efficient, environmentally friendly, and cost-effective technology for the extraction of protein from therapeutic leaves. Based on the ideal conditions of the small LBF system, a 98.36% protein recovery yield and a 79.12% separation efficiency were achieved. The upscaling study of this system exhibited the reliability of this technology for large-scale applications with a protein recovery yield of 99.44% and a separation efficiency of 93.28%. This technology demonstrated a simple approach with an effective protein recovery yield and separation that can be applied for the extraction of bioactive compounds from various medicinal-value plants.
Highlights
The usage of traditional herbs for preventive health care is widespread, and plants are the source of numerous natural antioxidants that could be utilized for the development of novel medicines
Formed using (NH4 )2 SO4 /ethanol was more efficient in recovering protein from kesum leaves than
The findings suggest that the efficiency of liquid biphasic flotation (LBF) in protein separation is dependent on the type of alcohol used in the system
Summary
The usage of traditional herbs for preventive health care is widespread, and plants are the source of numerous natural antioxidants that could be utilized for the development of novel medicines. Plants comprising high medicinal value are currently screened for a variety of pharmacological properties. Polygonum plant has been reported to contain a wide range of pharmacological properties and many studies have been conducted to evaluate the phytochemical and pharmacological aspects of the plant, which include anti-inflammatory activity [3], antiproliferative effects [4], anti-microbial activity [5], cytotoxic activity [6], gastric cytoprotective activity [7], and antiviral activity [8]. It has been proven that P. minus comprises many high-value components that include protein, flavonoids, and antioxidant vitamins, such as carotenes, retinol equivalents, and vitamin C. There are limited studies concerning the effective extraction techniques of the biomolecules from the plant extract
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