Apoptotic cell-treated dendritic cells induce immune tolerance through specifically facilitating development of CD4+ effector memory T cells (IRC10P.408)

Abstract

Abstract CD4+ memory T cells play an important role in induction of autoimmunity and chronic inflammatory responses. However, regulatory mechanisms of CD4+ memory T cell-mediated inflammatory responses are poorly understood. Here we showed that apoptotic cell-treated dendritic cells inhibit development and differentiation of CD4+ effector and central memory T cells in vitro and in vivo. Simultaneously, i.v. transfer of apoptotic T cell-induced tolerogenic dendritic cells can block development of experimental autoimmune encephalomyelitis (EAE), an inflammatory disease in central nervous system of C57BL/6J mouse. However, our results imply that it is effector memory CD4+ T cells, not central memory CD4+ T cells which play a major role in chronic inflammatory responses in mice with EAE. I.v. transfer of tolerogenic dendritic cells induced by apoptotic T cells leads to immune tolerance through specifically blocking development of CD4+ effector memory T cells compared with results of EAE control mice. Our results reveal a new mechanism of apoptotic cell-treated dendritic cell-mediated immune tolerance in vivo.