Aphids Associated With Caryophyllaceae in Iran With Description of a New Species (Hemiptera: Aphididae).

Although the high Arctic archipelago Svalbard is among the best-studied Arctic regions in terms of biodiversity, its aphid fauna is extremely limited. Two endemic species have been described to date, and no species of a foreign origin has been registered. Our observation is the first record of the non-native and potentially invasive green-peach aphid species Myzus (Nectarosiphon) persicae (Insecta, Hemiptera, Aphididae), which was collected on the largest island of the archipelago—Spitsbergen. The green-peach aphid was found on three different ornamental plants: Viola tricolor (Violaceae), Dianthus caryophyllus (Pink Kisses) (Caryophyllaceae) and Petunia × hybrida (surfinia) (Solanaceae). The plants were displayed in front of one of the shops in the main street of Longyearbyen, the largest populated settlement of Svalbard. The infected plants came from the only supermarket on the archipelago, which had brought them from the mainland of Norway. Although the aphids (winged and wingless viviparous females and immature ones) were clearly visible on all of the plants (the distinct colonies from few to numerous individuals), the owners were not aware that the plants were infected. Similarly, the supermarket staff who are responsible for the live green plants did not register the presence of aphids on the imported plants. In this context, our results also suggest that a stricter approach to regional biosecurity needs to be considered in order to avoid the risks of further unintentional introductions.

Diversity and geographic variation of endosymbiotic bacteria in natural populations of the pea aphid (Acyrthosiphon pisum) in China

Catchfly (Silene conoidea), an annual herb, is usually recognized as an emerging weed species in Eurasia and North America. The presence of somatic seed polymorphism might aid in the adaptation of this weed in different climatic conditions. We conducted laboratory and greenhouse experiments to study the seed polymorphism and influence of various environmental factors like temperature, salt stress, osmotic stress and burial depth on the germination and emergence characteristics of catchfly. Optimum germination of seeds of all colors was recorded at a temperature of 15 oC. Germination of catchfly seeds of all colors followed decreasing trend as NaCl concentration increased from 50 mM to 200 mM. Seed germination was maximum (87-96%) at 0 MPa but gradually decreased to 40% as osmotic stress increases up to -0.4 MPa and completely inhibited at 0.6 MPa of all seed colors. A slight increase (from 60 to 95%) in the germination of seeds of black and dark brown colors was observed when seeding depth increased from 0 to 2 cm but decreased when seeding depth increased from 2 to 4 cm in seeds of all colors. There was no emergence of catchfly at seeding depth of 6 cm or greater. Our results concluded that catchfly seeds have the potential to germinate and emerge in various environmental conditions, but germination/emergence percentage of seeds of all colors will be different in different environmental conditions. Soil amendments including deep ploughing may aid for the successful management of this weed in cultivated areas.

Aphid pathogens were collected in Australia, mainly from June 1977 until September 1979. A brief description is given of each of the six species of Entomophthora found; E. aphidis, E. exitialis, E. obscura. E. phalloides, E. planchoniana and E. virulenta. A key to these species is provided. The principal host aphids studied were two exotic legume aphids. Acyrthosiphon kondoi and Therioaphis trfolii f. maculata. In addition, pathogens were identified from Aphis craccivoua, Brevicoryne brassicae, Cliaetosiphon tetraihodum, Hyperoniyzus lactucae, Macrosiphum rosae, M. euplzorbiae, Myzus ornatus, M. persicae, Sitobion miscanthi, Rhopalosiphum rujabdorninalis, Acyrthosiphon pisum 'Broom-biotype' from Tasmania, Capitophorus elaeragni and three unidentified species. Three pathogens (E. exitialis, E. obscura and E. planchoniana) have been found causing epizootics in A. kondoi. Only one pathogen, E. obscura, occurs regularly in populations of T. trifolii f. maculata. The potential for introducing Entomophthora species for microbial control of aphids in Australia is discussed.

Many species of insects show phenotypically plastic dispersal traits in response to suboptimal environmental conditions. These polyphenetic traits can have large impacts on the population dynamics of the dispersing insects. Therefore, it is important to understand what environmental factors affect the development of dispersal traits and how these factors affect population dynamics via immigration/emigration. Pea aphids ( Acyrthosiphon pisum ) exhibit a polyphenetic wing dimorphism in which individuals are either winged (alate) or not (apterous). The production of alate offspring is determined by environmental cues experienced by the mother. Environmental factors associated with these cues have been intensively studied, with crowding, host plant quality, natural enemies, fungal infection, and viral infection being shown to affect the production of pea aphid alates under controlled laboratory conditions. Nonetheless, environmental factors affecting alate production have rarely been studied in field populations of pea aphids. Using data from a three‐year study of alate production in 5–9 alfalfa (lucerne) fields, we examined the effects of (i) pea aphid abundance, (ii) host plant maturity, (iii) temperature, (iv) predator and herbivore abundance, (v) parasitism, and (vi) entomopathic fungal incidence. In twice‐weekly samples taken over the growing season, alate production ranged from 0% to 83% of the nymphal population. Pea aphid abundance, temperature, and alfalfa maturity together explained 67% of the variation in alate production. The other factors we investigated explained little variation. These results suggest there is a limited number of key environmental factors that consistently predict changes in alate production in field populations, while many factors identified in lab studies may be unimportant. Our results highlight the value of investigating factors affecting the expression of plastic traits in insect species at a broad spatiotemporal scale and under natural conditions.

Parasitoid foraging behaviour is known to be influenced by interactions of genetic, physiological, environmental and experiential factors. Although the role of genetics, learning and conditioning in determining responses to foraging cues has been studied in lepidopteran parasitoids, aphid parasitoids have been less intensively researched. Using the tritrophic system,Vicia faba — Acyrthosiphon pisum — Aphidius ervi, evidence for the role of genetics and learning in parasitoid foraging is presented, and the difficulty of differentiating between genetic responses and those conditioned during parasitoid development is discussed. Aphidius ervi responds to aphid sex pheromones both in the field and in the laboratory. Since laboratory reared individuals have never experienced sexual aphids, the response must be genetic as it cannot have been conditioned during development. An example of a response conditioned during development is the variable response ofA. rhopalosiphi to different wheat cultivars depending upon host feeding. Aphid parasitoids also are adept at learning as shown by their responses to plant-derived cues which are learnt as Conditioned Stimuli (CS). Host products such as honeydew, as well as the host itself, can act as the Unconditioned Stimulus (US) in the learning process. Aphidius ervi offers a good model for investigating the role of these factors in parasitoid foraging behaviour. Finally, the value of such research for biological control programmes involving aphid parasitoids is discussed.

Abstract. We tested switching behaviour in four species of aphidiid parasitoids, using a two‐aphid experimental system consisting of second‐instar nymphs of pea aphid ( Acyrthosiphon pisum (Harris)) and alfalfa aphid ( Macrosiphum creelii Davis) feeding on broad beans in the laboratory. Aphidius ervi Haliday, A.pisivorus Smith, A.smithi Sharma & Subba Rao, and Pram pequodorum Viereck showed an innate preference for pea aphid when both host species were provided in equal numbers. Wasps encountered both aphid species equally but differed in their acceptance of alfalfa aphid. Females of A.pisivorus and P.pequodorum accepted alfalfa aphids when few pea aphids were available, but A. smithi always concentrated attacks on pea aphid. Aphidius ervi super‐parasitized an increasing proportion of pea aphids as their availability declined. Switching to the alfalfa aphid occurred in A.ervi and P.pequodorum (but not in A.pisivorus and A.smithi ) under the condition of a 1:3 ratio of pea aphids:alfalfa aphids. Wasps did not switch when more pea aphids than alfalfa aphids were provided (3:1 ratio). Alfalfa aphids were more likely than pea aphids to escape from parasitoid attack. Switching to the most abundant host may not be adaptive in these four species of aphid parasitoids. A foraging wasp incurs a potentially higher cost in lost opportunity time when attacking (and failing to oviposit in) alfalfa aphids. In addition, alfalfa aphids may have lower host quality than pea aphids, a difference that could influence offspring fitness.

The pea aphid is a global insect pest, and variable phenotypes can be produced by pea aphids in the same genotype in response to changes in external environmental factors. However, detailed dynamic gene regulation networks and the core markers involved in different biological processes of pea aphids have not yet been reported. In this study, we obtained the published genomic and transcriptomic data, and performed transcriptome profiling of five pea aphid morphs (winged asexual female, wingless asexual female, wingless sexual female, winged male and wingless male) from each of three pea aphid genotypes, i.e., the transcriptomes from a total of 15 types of pea aphids were analyzed and the type-specific expression of genes in five different morphs was identified. The expression profiling was verified by quantitative real-time PCR (qPCR) analysis. Moreover, we determined the expression features and co-expression networks of highly variable genes. We also used the ARACNe method to obtain 263 core genes related to different biological pathways. Additionally, eight of the identified genes were aligned with transcription factor families, indicating that they act as transcription factors and regulate downstream genes. Furthermore, we found reliable markers using random forest methodology to distinguish different morphs of pea aphids. Our study provides a systematic and comprehensive approach for analyzing the core genes that may play important roles in a multitude of biological processes from the insect transcriptomes.

Aphids are hemimetabolous insects that undergo incomplete metamorphosis without pupation. The annual life cycle of most aphids includes both an asexual (viviparous) and a sexual (oviparous) phase. Sexual reproduction only occurs once per year and is followed by many generations of asexual reproduction, during which aphids propagate exponentially with telescopic development. Here, we discuss the potential links between viviparous embryogenesis and derived developmental features in the pea aphid Acyrthosiphon pisum, particularly focusing on germline specification and axis determination, both of which are key events of early development in insects. We also discuss potential evolutionary paths through which both viviparous and oviparous females might have come to utilize maternal germ plasm to drive germline specification. This developmental strategy, as defined by germline markers, has not been reported in other hemimetabolous insects. In viviparous females, furthermore, we discuss whether molecules that in other insects characterize germ plasm, like Vasa, also participate in posterior determination and how the anterior localization of the hunchback orthologue Ap-hb establishes the anterior-posterior axis. We propose that the linked chain of developing oocytes and embryos within each ovariole and the special morphology of early embryos might have driven the formation of evolutionary novelties in germline specification and axis determination in the viviparous aphids. Moreover, based upon the finding that the endosymbiont Buchnera aphidicola is closely associated with germ cells throughout embryogenesis, we propose presumptive roles for B. aphidicola in aphid development, discussing how it might regulate germline migration in both reproductive modes of pea aphids. In summary, we expect that this review will shed light on viviparous as well as oviparous development in aphids.

The pea aphid, Acyrthosiphon pisum, maintains extreme variation in resistance to its most common parasitoid wasp enemy, Aphidius ervi, which is sourced from two known mechanisms: protective bacterial symbionts, most commonly Hamiltonella defensa, or endogenously encoded defences. We have recently found that individual aphids may employ each defence individually, occasionally both defences together, or neither. In field populations, Hamiltonella-infected aphids are found at low to moderate frequencies and while less is known about the frequency of resistant genotypes, they show up less often than susceptible genotypes in field collections. To better understand these patterns, we sought to compare the strengths and costs of both types of defence, individually and together, in order to elucidate the selective pressures that maintain multi-modal defence mechanisms or that may favour one over the other. We experimentally infected five aphid genotypes (two lowly and three highly resistant), each with two symbiont strains, Hamiltonella-APSE8 (moderate protection) and Hamiltonella-APSE3 (high protection). This resulted in three sublines per genotype: uninfected, +APSE8 and +APSE3. Each of the 15 total sublines was first subjected to a parasitism assay to determine its resistance phenotype and in a second experiment, a subset was chosen to compare fitness (fecundity and survivorship) in the presence and absence of parasitism. In susceptible aphid genotypes, parasitized sublines infected with Hamiltonella generally showed increased protection with direct fitness benefits, but clear infection costs to fitness in the absence of parasitism. In resistant genotypes, Hamiltonella infection rarely conferred additional protection, often further reduced fecundity and survivorship when enemy challenged, and resulted in constitutive fitness costs in the absence of parasitism. We also identified strong aphid genotype×symbiont-strain interactions, such that the best defensive strategy against parasitoids varied for each aphid genotype; one performed best with no protective symbionts, the others with particular strains of Hamiltonella. This surprising variability in outcomes helps explain why Hamiltonella infection frequencies are often intermediate and do not strongly track parasitism frequencies in field populations. We also find that variation in endogenous traits, such as resistance, among host genotypes may offer redundancy and generally limit the invasion potential of mutualistic microbes in insects.

Journal Article Sexual and Fundatrix-Like Morphs in Asexual Australian Populations of the Pea Aphid (Homoptera: Aphididae) Get access P. A. Mackay, P. A. Mackay 1 Division of Entomology, CSIRO, Canberra, A.C.T. 2601, Australia 1 Current address: Department of Entomology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. Search for other works by this author on: Oxford Academic PubMed Google Scholar R. J. Lamb, R. J. Lamb 2 Division of Entomology, CSIRO, Canberra, A.C.T. 2601, Australia 2 Current address: Agriculture Canada, 195 Dafoe Road, Winnipeg, MB R3T 2M9, Canada. Search for other works by this author on: Oxford Academic PubMed Google Scholar M. A. Hughes M. A. Hughes Division of Entomology, CSIRO, Canberra, A.C.T. 2601, Australia Search for other works by this author on: Oxford Academic PubMed Google Scholar Environmental Entomology, Volume 18, Issue 1, 1 February 1989, Pages 111–117, https://doi.org/10.1093/ee/18.1.111 Published: 01 February 1989 Article history Received: 29 December 1987 Accepted: 01 September 1988 Published: 01 February 1989

1. Selection does not only operate in a genotype (G) × environment (E) context, but can also be modulated by the activities of organisms interacting with their environment (G × G × E). 2. The influences of aphid clonal identity and host plant (Vicia faba) intraspecific genetic variation on the performance of five genotypes of pea aphid (Acyrthosiphon pisum) were investigated – with and without interaction with a competing heterospecific clone of vetch aphid (Megoura viciae) – across three cultivars of V. faba. 3. Pea aphid performance in the presence of a competing vetch aphid clone (G × G × E) compared with the absence of competition (G × E) revealed strong context-dependent, genotype-specific shifts in performance, influenced by plant cultivar, competitor presence and their interaction. 4. The performance of vetch aphid in competition with each pea aphid clone was also compared. Here, competitor's genotype and abundance underlay a remarkably varied response by vetch aphid across interactions. 5. The study shows that aphid genotypes exhibit a varying degree of risk spreading, contingent on competitor identity and the patterns of aggregation across three plant cultivars. Owing to feedback loops between species activities and selective forces acting on them, our findings suggest that there are context-dependent responses by competitors that are shaped via the interplay of the co-occurring species and their biotic environment. 6. This work highlights the complexity of species interactions and the importance of investigating reciprocity between competition and intraspecific genetic variation. A better understanding of the eco-evolutionary interactions between phloem-feeding insects and their host plants can potentially be used to enhance crop protection and pest control.

The adaptability of insects to hosts has long been a focal point in the study of insect-plant interactions. The pea aphid (Acythosiphon pisum), a significant pest of numerous leguminous crops, not only inflicts direct economic losses but also disseminates various plant viruses. To understand how pea aphids adapt to diverse alfalfa varieties. We analyzed the differentially expressed genes (DEGs) of pea aphids in distinct alfalfa varieties using transcriptome sequencing, and subsequently conducted functional validation of these genes. Comparative analysis between pea aphids feeding on susceptible and resistant strains revealed that DEGs in aphids feeding on resistant strains were primarily associated with transcriptional enrichment in the sugar, amino acid, protein, and lipid metabolism pathways. Fourteen DEGs related to adaptation of the pea aphid to alfalfa were chosen, including five carboxylesterases (CarE), four cytochrome P450s, three glutathione S-transferases, and two peroxidases (POD). RT-qPCR results indicated significant up-regulation of two carboxylesterase genes and two peroxidase genes after 24 h of feeding resistant alfalfa (Gannong 5, GN5) compared to the susceptible varieties (Hunter River, LRH), particularly highlighting the high expression levels of ApCarE4 and ApPOD3. Simultaneously, RNAi-induced knockdown of ApCarE4 and ApPOD3 led to a higher mortality of pea aphids in the alfalfa Hunter River. These results indicate that ApPOD3 and ApCarE4 are involved in the detoxification of metabolic functions in the adaptation of pea aphids to host switching. These findings contribute to the understanding of pea aphid adaptation to host plants and lay a foundation for further exploration of the physiological roles of carboxylesterase and peroxidase genes in pea aphids.

Simple SummaryThe decrease of stratospheric ozone contributes to a significant increase in solar ultraviolet (UV-B) radiation. This effect has led to investigation on the impact of increased UV-B radiation on insect physiology. Pea aphid is a worldwide important agricultural pest and is difficult to control due to its small size, high fecundity, and non-sexual reproduction. As such, there is a need for study of the effects of UV-B radiation on aphid physiology, to raise awareness of the mechanisms of aphid responses to UV-B stress. The results reported here revealed that UV-B radiation can lead to an increase in sugar contents in both red and green morphs of this aphid species, and confirmed the effects of UV-B radiation on aphid physiology by means of influencing protective enzyme activity.Natural and anthropogenic changes have been altering many environmental factors. These include the amount of solar radiation reaching the Earth’s surface. However, the effects of solar radiation on insect physiology have received little attention. As a pest for agriculture and horticulture, aphids are one of the most difficult pest groups to control due to their small size, high fecundity, and non-sexual reproduction. Study of the effects of UV-B radiation on aphid physiology may provide alternative control strategies in pest management. In this study, we examined the effects of UV-B radiation on protein and sugar contents, as well as the activities of protective enzymes, of the red and green morphs of the pea aphid over eight generations. The results indicated a significant interaction between UV-B radiation and aphid generations. Exposure of the pea aphids to UV-B radiation caused a significant decrease in the protein content and a significant increase in the glycogen and trehalose contents at each generation as measured in whole aphid bioassays. The enzyme activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) of the pea aphids changed significantly at each generation with UV-B treatments. The SOD activity increased over eight generations to the highest level at G7 generation. However, the enzyme activity of CAT first increased and then decreased with UV-B treatments, and POD mostly gradually decreased over the eight generations. Therefore, UV-B radiation is an environmental factor that could result in physiological changes of the pea aphid. Moreover, our study discovered that red and green aphids did not display a significant consistent difference in the response to the UV-B treatments. These results may prove useful in future studies especially for assessing their significance in the adaptation and management against UV-B radiation.

Simple SummaryThe pea aphid, Acyrthosiphon pisum, is a major agricultural pest. It uniquely possesses fungal-derived genes that allow it to produce carotenoid pigments typically found in plants. Our study reveals a previously unknown role for these pigments beyond coloration. Under cold stress (12 °C), high light intensity triggers carotenoids to act like micro solar panels, capturing light to fuel ATP production. This leads to a striking 240% boost in energy, enhancing aphid development and reproduction under cold conditions. In contrast, the system remains inactive at warmer temperatures (22 °C). These findings demonstrate how a single horizontal gene transfer event can provide an organism with a novel energy-capturing ability, markedly increasing its adaptability in variable environments.The pea aphid, Acyrthosiphon pisum, possesses horizontally acquired fungal carotenoid biosynthesis genes, enabling de novo production of carotenoids. Although carotenoids are known to contribute to photo-protection and coloration, their potential role in energy metabolism and population fitness under thermal stress is still unclear. This study investigated the interactive effects of temperature and light intensity on energy homeostasis and life-history traits in A. pisum. Using controlled environmental regimes, we demonstrate that light intensity significantly influenced the ATP content, development, and reproductive output of A. pisum at 12 °C, but not at 22 °C. Under cold stress (12 °C), high light intensity (5000 lux) increased ATP content by 240%, shortened the pre-reproductive period by 46%, extended reproductive duration by 62%, and enhanced the net reproductive rate (R0) and intrinsic rate of increase (rₘ) compared to low light intensity (200 lux). These effects were abolished at the optimal temperature (22 °C), indicating a temperature-gated, light-dependent mechanism. Demographic analyses revealed that carotenoid-associated solar energy harvesting significantly improves fitness under cold conditions, likely compensating for metabolic depression. Our findings reveal a novel ecological adaptation in aphids, where horizontally transferred genes may enable light-driven energy supplementation during thermal stress. This study provides new insights into the physiological mechanisms underlying insect resilience to climate variability and highlights the importance of light as a key environmental factor in shaping life-history strategies in temperate agroecosystems.