Abstract
Previously we reported that G protein-coupled receptor APJ endogenous ligand apelin-13 induced adhesion of monocytes to human umbilical vein endothelial cells(HUVECs).Now we investigated whether phosphatidylinositol 3-kinase(PI3K) signaling pathway mediated monocytes(MCs) adhesion to HUVECs induced by apelin-13.Human umbilical vein endothelial cell line ECV304 was cultured in DMEM medium and the monocyte cell line THP-1 was cultured in 1640 medium.Myeloperoxidase(MPO) assay was used to identify effects of monocytes adhesion to HUVECs.Expression of vascular cell adhesion molecule(VCAM)-1,phospho-PI3K and PI3K were detected by Western blotting.Apelin-13 promoted PI3K phosphorylation in concentration-dependent and time-dependent manner,which reached the peak at 1 μmol/L and 30 min respectively.The PI3K inhibitor LY294002 inhibited PI3K phosphorylation and the expression of VCAM-1 in HUVECs induced by apelin-13.And the PI3K inhibitor LY294002 inhibited the MCs adhesion to HUVECs induced by apelin-13.It can be concluded that apelin-13 promoted monocytes adhesion to HUVECs through VCAM-1 mediated by PI3K signaling pathway.
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