Abstract
Antiviral effects of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP, and ribavirin on double-stranded DNA Cauliflower mosaic virus (CaMV) were evaluated in Brassica pekinensis plants grown in vitro on liquid medium. A double-antibody sandwich ELISA was used for relative quantification of viral protein and PCR for detection of CaMV nucleic acid in plants. Ribavirin and PMEA had no significant antiviral effect. (S)-HPMPC at concentration 50 mg l−1 and PMEDAP at concentrations 50 and 12.5 mg l−1 significantly (P < 0.05) reduced CaMV concentration in plants within 42–63 days to levels detectable neither by ELISA nor by PCR. A phytotoxicity experiment resulted in progressive yellowing of leaves and dwarfing in plants cultured 42 days on media with concentrations 12.5, 25 and 50 mg l−1 of (S)-HPMPC and PMEDAP. Reduction in fresh and dry weights of plants was significant (P < 0.05) already at 12.5 mg l−1 with both compounds.
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