Abstract
<p><strong>Objective: </strong>The aim of this study was to investigate the capability of <em>Zizyphus spina christi</em> methanol extract to inhibit cancer cell line proliferation.</p><p><strong>Methods: </strong>The leaves of <em>Zizyphus spina christi</em> were extracted by cold maceration method. The anti-proliferative activity of the methanol extract against rhabdomyosarcoma (RD) cell line was tested by 3-(4, 5 Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemical constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant activity was assessed by measuring free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. <strong></strong></p><p><strong>Results: </strong>The percentage extraction yield for leaves with methanol was 20.64%. The methanol extract showed dose dependent inhibition of RD cell line, the IC<sub>50</sub> was 154.44 µg/ml. GC-MS showed the presence of flavonoid fraction and other compounds with antioxidant activity. The methanol extract demonstrated DPPH scavenging activity with IC<sub>50</sub> of 33.91 mg/ml.</p><p><strong>Conclusion: </strong>Methanol extract showed<strong> </strong>potential anti-proliferative activity against RD cell line,<strong> </strong>which could be due to its antioxidant activity.</p>
Highlights
Cancer is one of the most leading causes of mortality in the world, accounting for almost 13% of deaths worldwide [1]
Methanol extract showed potential anti-proliferative activity against RD cell line, which could be due to its antioxidant activity
The results showed that all the tested concentrations of the methanol extract significantly inhibited RD cell line growth after 48 h (P0.05)
Summary
Cancer is one of the most leading causes of mortality in the world, accounting for almost 13% of deaths worldwide [1]. Rhabdomyosarcoma (RMS) is a malignancy that arises from skeletal muscle precursors [3]. It is the most common type of soft tissue sarcoma in children and adolescents less than twenty years old [4]. Chemotherapy is a major mode of treatment for various cancers. It is usually associated with adverse side effects, ranging from nausea to bone marrow failure [5], and development of multidrug resistance (MDR). Finding natural compounds from plants may provide an alternative cancer treatment [6]
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