Abstract

We investigated the effects of dietary Romet®30 (sulfadimethoxine–ormetoprim (SDMX–OMP)) on Cryptocaryon irritans infection in red sea bream Pagrus major and tiger puffer Takifugu rubripes. In Experiment I, 100% mortality of P. major due to C. irritans infection was observed at 10days and 21days after exposure to theronts (infective stage) in the a control group without Romet®30 and the group treated with 50mg Romet®30/kg body weight (BW)/day for 14days, respectively. Thus, mortality in the treated group was markedly delayed compared with that in the control group. In Experiment II, 100% mortality of P. major in the control group due to C. irritans infection was recorded at 11days after exposure to theronts. In contrast, mortality due to parasite infection was not observed in the group treated with Romet®30 at 50mg/kg BW/day for 14days, and no parasites were found in any surviving fish after 33days exposure. In addition, the number of parasites on the gills of T. rubripes treated with 50mg Romet®30/kg BW/day for 14days was significantly lower than that in the control group after 16days exposure. These results show that in-feed Romet®30 at 50mg/kg BW/day for 14days had antiparasitic and therapeutic effects against C. irritans in both P. major and T. rubripes. Thus, dietary Romet®30 could be useful for controlling C. irritans infection.

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