Abstract
(1) Background: Extensive research has focused on flavan-3-ols, but information about the bioactivities of green tea flavonols is limited. (2) Methods: In this study, we investigated the antioxidative, anti-inflammatory, and anticancer effects of flavonol glycosides and aglycones from green tea using in vitro cell models. The fractions rich in flavonol glycoside (FLG) and flavonol aglycone (FLA) were obtained from green tea extract after treatment with tannase and cellulase, respectively. (3) Results: FLG and FLA contained 16 and 13 derivatives, respectively, including apigenin, kaempferol, myricetin, and quercetin, determined by mass spectrometry. FLA exhibited higher radical-scavenging activity than that of FLG. FLG and FLA attenuated the levels of intracellular oxidative stress in neuron-like PC-12 cells. The treatment of RAW 264.7 murine macrophages with FLG and FLA significantly reduced the mRNA expression of inflammation-related genes in a dose-dependent manner. Furthermore, FLG and FLA treatments decreased the viability of the colon adenoma cell line DLD-1 and breast cancer cell line E0771. Moreover, the treatment with FLG or FLA combined with paclitaxel had synergistic anticancer effects on the DLD-1 cell line. (4) Conclusions: Flavonols from green tea exerted beneficial effects on health and may be superior to flavan-3-ols.
Highlights
Green tea is a familiar beverage with high amounts of bioactive polyphenols, such as catechins, flavones, and flavonols [1]
16 derivatives of apigenin, kaempferol, myricetin, and quercetin were identified in flavonol glycoside (FLG) and 13 in flavonol aglycone (FLA)
We concluded that FLG and FLA without catechins from green tea have potent antioxidant capacities and reduce oxidative stress in PC-12 cells
Summary
Green tea is a familiar beverage with high amounts of bioactive polyphenols, such as catechins (flavan-3-ols), flavones, and flavonols [1]. Flavonols and flavones in green tea include myricetin, quercetin, apigenin, and kaempferol [3]. These green tea flavonols and flavones are mainly in the form of glycosides, and their content and compositions vary among tea cultivars [4,5]. Flavonols exhibit anticancer activity by inhibiting the proliferation and angiogenesis of cancer cells and exerting pro-apoptotic effects [6]. These anticancer effects have been demonstrated in several cancer cell lines, in addition to antioxidative and antihyperlipidemic effects [6,7,8]. The effects of flavonol supplementation on cardiometabolic risk factors revealed significant reductions in triacylglycerol, total cholesterol, low-density lipoprotein, and fasting plasma glucose levels, as well as blood pressure, and a Antioxidants 2019, 8, 278; doi:10.3390/antiox8080278 www.mdpi.com/journal/antioxidants
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
