Antioxidant and immune defences of rainbow trout (Oncorhynchus mykiss) offered plant oils differing in fatty acid profiles from early stages

Abstract

The antioxidant defence and immune response of rainbow trout (Oncorhynchus mykiss) that had received plant oils, rich in either n-3 polyunsaturated fatty acids (PUFA; linseed oil) or n-6 PUFA (safflower oil) was evaluated upon antigen exposure. The fish employed in this study had been offered the diets for 18 months from the first feeding. Rainbow trout from each group were injected intraperitoneally with formalin-killed bacteria (Aeromonas hydrophila) or were sham-injected and observations were made 24 h later. Though the fish fed safflower oil seemed to be under relatively greater oxidative stress, the antioxidant defences (superoxide dismutase, catalase, glutathione peroxidase) were as effective as in those fed linseed oil. The humoral (alternate complement activity and lysozyme activity) and cellular (phagocytic activity and lymphocyte proliferation) immune responses were not significantly affected by the oil offered. With the exception of reactive oxygen production that was significantly greater in the linseed oil fed fish, both groups did not differ greatly in their immune responses after antigen exposure. Thus, fish fed safflower oil that was deficient in n-3 PUFA was able to sustain most of the critical responses similar to those fed linseed oil suggesting that plant oils of both fatty acid categories were effective for this fresh water fish.