Abstract
Background and Aims: Free-radical-mediated peroxidation of membrane lipids and oxidative damage of DNA and proteins are believed to be associated with a variety of chronic pathological complications such as cancer. The aim of this study was to describe antioxidant and anti-inflammatory of silver nanoparticles (AgNPs) synthesized using medicinal plant extract of Sage (Salvia officinalis). Materials and Methods: AgNPs were synthesized using S. officinalis as reducer agents and characterized using ultraviolet-visible, Fourier transform infrared spectroscopy, particle seizer, and transmission electron microscopy. Toxicity of AgNPs on MCF-7 cells was investigated using 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Changes in expression of inflammation related genes include cyclooxygenase-2, IL-8, and tumor necrosis factor-alpha (TNF-α) genes were evaluated using semi-quantification reverse transcription-polymerase chain reaction (RT-PCR). The antioxidant potential of capped AgNPs was assessed using 1,1 diphenyl-2-picryl-hydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radicals activity assay. Results: AgNPs successfully synthesis with an average size of 16 nm and spherical. FTIR spectrum from plant extract and AgNPs indicated the extract covered nanoparticles. AgNPs decreased cells viability with inhibitory concentration (IC50) of 25 μg/ml and 20 μg/ml after 24 and 48 h, respectively. To ascertain the anti-inflammatory genes expression, MCF-7 cells were treated with 20 μg/ml AgNPs (concentration below of IC50 value according to MTT assay). Semi-quantification RT-PCR results showed that AgNPs increased IL-8 and TNF-α genes expression 28.76% and 42%, respectively, but suppressed cyclooxygenase-2 gene expression with 20.5% comparing to control groups. Antioxidant assay of green synthesized AgNPs coated by S. officinalis extract showed free radical scavenging effect with IC50 of 830 and 800 μg/ml for DPPH and ABTS radicals, respectively. Conclusion: The coated AgNPs with S. officinalis have promising potential as a source for the development of chemotherapeutic agents in future.
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