Abstract
Antioxidants are substances that can provide endogenous protection and exogenous oxidative stress by capturing free radicals. Many plants are efficacious as antioxidants, namely plants that contain polyphenols, especially flavonoids, so many are formulated as natural antioxidants. Plants such as Muntingia calabura, Syzygium cumini, Ocimum basilicum, and Eleutherine bulbosa contain polyphenol compounds, especially flavonoids which are efficacious as natural antioxidants. This research aimed to study antioxidant activity derived from some potential plants using the DPPH method by calculating the IC50 value of each plant extract. This research method starts from the determination process to prove the validity of the plants used, the extraction process using the maceration method with 70% ethanol solvent, then the antioxidant activity of extracts from each plant was carried out using the DPPH method. This research starts from the determination process to ensure the correctness of the plants used, then the extraction process is carried out using the maceration method with 70% ethanol solvent. After that the antioxidant activity was determined from the four plants using the DPPH method to see the strongest IC50 value among the four plants. IC50 is the concentration of the sample to inhibit 50% of free radicals. The results of IC50 values from ethanol extract of M. calabura leaves, Syzygium cumini leaves, Ocimum basilicum leaves and Eleutherine bulbosa bulbs, were 18.72; 63,84; 141.59 and 173.15 ppm. Ethanol extract of M. Calabura has a smaller IC50 value of 18.72 ppm which has a very strong and most powerful antioxidant from the ethanol extract of Syzygium cumini, Ocimum basilicum and Eleutherine bulbosa. Keywords : Antioxidant, Muntingia calabura, Syzygium cumini, Ocimum basilicum, Eleutherine bulbosa, DPPH Method
Highlights
Antioxidants defined as molecules having ability to inhibit the oxidation of other molecules mostly caused by free radicals, can reduces the damages due to oxygen [1,2,3]
This research aims to determine the antioxidant activity of Muntingia calabura leaves, Syzygium cumini leaves, Ocimum basilicum leaves and Eleuherine bulbosa bulbs since they contain polyphenol compounds, especially flavonoids which known to have efficacious as natural antioxidants
Sample Preparation Ethanol extracts were prepared with a stock solution of 1000 ppm. 1000 ppm stock solution is diluted to concentration 0, 5, 10, 15, 20 and 25 ppm for Muntingia calabura L., 0, 20, 40, 60, 80 and 100 ppm for Syzigium cumini L., 0, 50, 100, 150, 200 and 250 ppm for Ocimum basilicum L. and Eleutherine bulbosa (Mill.)
Summary
Antioxidants defined as molecules having ability to inhibit the oxidation of other molecules mostly caused by free radicals, can reduces the damages due to oxygen [1,2,3]. Antioxidants can be catagorized as either synthetic or natural and both are included in formulations. Synthetic antioxidants (e.g. butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and propyl gallate) has been largely used for many purposes since the ease of production leading to lower prices. High potential health risks following a high consumption of synthetic antioxidants has been reported by some studies. Despite the high demand for synthetic antioxidants by market, concerns on natural antioxidants have been increased in the last few years and it is expected to continue and growing. The consumer prefer organic and natural products, since they use less additives it is hoped to have less side effects than synthetic ingredients[4]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
