Abstract
A broth microdilution technique was used to determine the antimicrobial susceptibility of 15 field isolates of Mycoplasma hyorhinis to 10 antimicrobial agents, representative of different classes, and contrasting newer agents to existing ones. For the macrolides, the MIC 90 for tylosin and tilmicosin was 1 and 4 μg/ml, respectively, but was ≥16 μg/ml for erythromycin. Tetracycline, lincomycin and enrofloxacin each had an MIC 90 of 2 μg/ml. The mycoplasma had similar levels of susceptibility to the aminoglycoside and aminocyclictol classes exhibiting an MIC 90 of 4 μg/ml for gentamicin and 2 μg/ml for spectinomycin. The isolates exhibited high MICs to trimethoprim/sulfamethoxazole with an MIC 90≥16/304 μg/ml. In summary, M. hyorhinis isolates from the US had low MICs against a variety of antimicrobials tested, with the exception of erythromycin and trimethoprim/sulfamethoxazole.
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