Antimicrobial Resistance Patterns of Urine Culture in Tertiary Care Teaching Hospital Islamabad, Pakistan

Abstract

Background: Urine cultures are performed in laboratories to identify any bacteria or other microorganisms present in urine samples. The purpose of this study was to investigate the microorganisms growing resistant to antibiotics in the urine culture and their antibiotics susceptibility testing. Methods: For this investigation, the Microbiology Department's hospital management information systems records provided the culture and sensitivity data of the uropathogens found in urine samples. Urine samples collected midstream were prepared for microscopy and culture, and the organisms were identified using globally accepted methods. The Kirby-Bauer disk diffusion method was used to determine antibiotic susceptibility in accordance with hospital clinical and laboratory standards as well as international protocols. In this investigation, the information and data were assessed using descriptive statistics. Results: A total number of 207 urine culture data were extracted from the hospital management information system records of which, 116 samples showed positive bacterial growth. Candida had positive growth in 16.4%. Out of positive samples gender-wise, females were in the majority 66.4% and males were 33.6%. Escherichia coli bacteria frequently isolated in positive samples 58/116 (50%), whereas, Enterococcus spp were positive in 17 patients out of 116 (14.7 %). Gram-negative rods were positive in 16 patients out of 116 (13.8%), Klebsiella spp were positive in 4 patients out of 116(3.4%) and Pseudomonas aeruginosa were least positive in only 2 patients out of 116 positive samples (1.7 %). Conclusion: In our study according to gender distribution female frequency was higher than males. Escherichia coli bacteria were the most common isolate in positive samples, whereas, Enterococcus spp were observed. Vancomycin was most sensitive in all urine cultures against all organisms and Erythromycin was most resistant in urine culture. Antimicrobial resistance is a serious subject on national and international levels that needs continued surveillance and resolute management.