Abstract
The flavonoid fraction of bergamot juice (BJe) has demonstrated radical-scavenging and anti-inflammatory activities. The aim of this study was to investigate the effects of BJe on the modulation of the ileum inflammation caused by intestinal ischemia/reperfusion (I/R) injury. This was induced in mice by clamping the superior mesenteric artery and the celiac trunk for 30 min, followed by release of the clamp, allowing reperfusion for 1 h. After 1 h of reperfusion, animals were killed for histological examination of the ileum tissue, the expression of proinflammatory cytokines and adhesion molecules; for biochemical assays such as myeloperoxidase activity (MPO) and malondialdehyde (MDA) levels. Moreover, by Western blot analysis, we also investigated the expression of nuclear factor NF-κB, IκB-α, inducible nitric oxide synthase (iNOS) and the expression of antioxidant such as MnSOD. The results obtained from histological and molecular examinations showed that BJe was able to reduce histological damage, cytokines production, adhesion molecules expression, neutrophil infiltration and oxidative stress. In addition, NF-κB and MAP kinases pathways were also modulated by BJe treatment. In conclusion, in this study we demonstrated that BJe could represent a possible treatment against secondary events of intestinal I/R injury.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
