Anti-human acute leukemia, cytotoxicity, antioxidant, and collagenase and aldose reductase inhibition effects of 2′-Hydroxy-4′,5′-dimethoxyacetophenone in the in vitro condition

Abstract

IntroductionIn this study, it is recorded the inhibition effect of 2′-Hydroxy-4′,5′-dimethoxyacetophenone on aldose reductase and collagenase enzymes. Also, we have investigated that the in vitro inhibition effects of 2′-Hydroxy-4′,5′-dimethoxyacetophenone on aldose reductase and collagenase enzymes.Material and methodsTo investigate the antioxidant effects of 2′-Hydroxy-4′,5′-dimethoxyacetophenone, the DPPH test was used in the presence of butylated hydroxytoluene as the positive control. MTT test was used on normal (HUVEC) and human acute leukemia (32D-FLT3-ITD, Human HL-60/vcr, MOLT-3, and TALL-104) cell lines. 2′-Hydroxy-4′,5′-dimethoxyacetophenone had high cell death and anti-human acute leukemia effects against 32D-FLT3-ITD, Human HL-60/vcr, MOLT-3, and TALL-104 cell lines.ResultsThe 2′-Hydroxy-4′,5′-dimethoxyacetophenone inhibited half of the DPPH molecules in the concentration of 157 µg/mL. Among the above cell lines, the best result of anti-human acute leukemia properties of silver nanoparticles was gained in the cell line of UM-UC-3. The results of this study indicated the excellent anti-human acute leukemia potentials of 2′-Hydroxy-4′,5′-dimethoxyacetophenone in the in vitro condition.ConclusionsAfter confirming the above results in the clinical trial researches, this formulation may be administrated for the treatment of several types of acute leukemia in humans. After that, the comparison of the biological activities of the 2′-Hydroxy-4′,5′-dimethoxyacetophenone molecule against the studied enzymes was done by molecular docking method.