Abstract
Changes in the antigenic content of resting cysts of ciliates after freezing at −196 °C have been studied. The results obtained from experiments with native immunosera using the immobilizing and agglutinating activity allow the conclusion that freezing causes significant changes in the cyst surface antigens. Ultraviolet irradiation did not cause any notable shifts in the surface antigen composition. By means of immunodiffusion methods, changes in the soluble antigen set of cysts after freezing have been demonstrated. Freezing induces a specific simplification in the antigen spectrum and a new antigen appearance characteristic of cysts subjected to freezing.
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