Antifungal activity of Ventilago maderspatana on Trichophyton rubrum - An in vitro study

Abstract

The aim of the study was to contribute a single drug for specific dermatophyte species to encourage diagnosis specific treatment. The study had two arms one clinical part consisting of cross-sectional study of 80 participants and other part invitro study. The survey was conducted to know the prevalence of dermatophyte species among cutaneous dermatophytosis patients in Kottakkal population there by collecting the skin scrapings of the patients. As the second part of the study these skin scrapings were cultured in sabouraud dextrose agar (SDA) and the species were inoculated from their and identified. Thus from 80 participants 2-genus and 6 different species were obtained. Trichophyton and microsporum were the genus and among Trichophyton 4 species like T.rubrum, T.mentagrophytes, T.tonsurans and T.verrucossum and Microsporum genus consists of M.canis and Microsporumaudouinii. First MIC was calculated and after that antifungal activity was done through the agar well diffusion method using ventilago maderaspattana extracts against fluconazole. Three different extracts were used chloroform, methanol and ethanol. In this article the action of easily available low polarity chloroform extract against the most prevalent species T. rubrum is highlighted because it shows the highest mean zone of inhibition in agar well diffusion method.