Abstract
BackgroundUraria picta (Jacq.) DC is a traditional Indian herb used in the Ayurvedic system of medicine. It is an ingredient of the popular Ayurvedic formulation “Dasamoola.” It is also traditionally used as an anti-inflammatory herb in Ayurveda. The plant also has excellent antioxidant potential. Different parts of Uraria picta are useful and have diverse medicinal properties. The present investigation was done to evaluate the anticancer activity of methanolic extract of aerial parts of Uraria picta (Jacq.).ResultsPreliminary phytochemical screening of MEUP confirmed the presence of flavonoids, phenolics, glycosides, and tannins. Total phenolic and flavonoid contents in MEUP were found to be 14.6 gram equivalent of gallic acid and 0.735 gram equivalent of catechin, respectively. IC50 values of cisplatin and MEUP were found to be 8.75 μg/ml and 436.92 μg/ml, respectively. Induction of apoptosis was evident from DAPI staining showing a dose-dependent increase in apoptosis. Also, a reduction in intracellular ROS was evident from the DCFH-da assay, where a dose-dependent decrease in intracellular ROS (fluorescence) was observed. Significant reduction (P < 0.05) in tumor volume was observed in mice receiving cisplatin and MEUP at both dose levels.ConclusionThe methanolic extract of aerial parts of Uraria picta (Jacq.) DC is effective in inhibiting tumor growth and has significant anticancer property.
Highlights
Uraria picta (Jacq.) disease control (DC) is a traditional Indian herb used in the Ayurvedic system of medicine
Cell line and animals The A549 human lung cancer cell line was obtained from the National Centre for Cell Science (NCCS), Pune, Maharashtra, and was maintained at 37 °C in RPMI1640 medium supplemented with 10% Fetal bovine serum (FBS) under 5 % CO2 and humidified condition in a CO2 incubator [19, 20]
An IC50 value of 436.92 μg/ml is too high to establish the crude methanolic extract of Uraria picta as a potential anticancer agent, the results from other in vitro and in vivo experiments conducted in our study clearly indicate that the plant has promising anticancer properties
Summary
Uraria picta (Jacq.) DC is a traditional Indian herb used in the Ayurvedic system of medicine. The present investigation was done to evaluate the anticancer activity of methanolic extract of aerial parts of Uraria picta (Jacq.). Cancer accounts for 9.6 million deaths worldwide in the year 2018. Around 18.1 million new cases of cancer occurred in the same year, making it a leading cause of deaths globally [2]. In the USA, cancer stands as the second most common cause of deaths after cardiovascular diseases, responsible for around 25% of total deaths per year [3, 4]. Natural products and secondary metabolites isolated from plant sources are considered to be less toxic to normal cells [7]. A majority of anticancer agents that are employed in the present scenario for the treatment of cancer are obtained from plant sources. Data from FDA reports suggest that 40% of approved anticancer molecules are natural compounds and a majority of them are presently utilized in anticancer therapy [7, 8]
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