Abstract
An assay for cell-mediated lysis of sheep erythrocytes has been developed using technetium-99m (99mTc) as a radioisotopic label. Spleen cells obtained from sensitized BALB/c mice in combination with either non-immune or immune mouse anti-sheep RBC serum produced maximum release (100%) of 99mTc. Non-immune spleen cells in combination with immune serum produced significantly greater release of 99mTc compared to that observed with non-immune cells in the presence of non-immune serum. The kinetics of antibody-mediated complement dependent release of 99mTc as a function of time and temperature paralleled the release of 51Cr and hemoglobin as an indicator of erythrocyte membrane injury. Because of its high specific activity, low spontaneous release, and ready availability in those institutions where diagnostic scanning is performed, 99mTc appears to be particularly well suited for the assessment of both antibody and cell-mediated lysis of erythrocytes.
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