Antibiotic resistance of enterobacteria isolated from freshwater bodies of different climatic zones

Abstract

An important problem of our time is the resistance of bacteria to antimicrobial drugs. Surface water bodies accumulate all kinds of antibiotic-resistant bacteria found in the catchment area.The aim. To compare the antibiotic resistance of enterobacteria isolated from freshwater ecosystems of the Murmansk and Ryazan regions.Methods. Isolation was performed by the disk-diffusion method. For species identification, the “Rapid-entero 200 M” test system was used. Sensitivity was determined to 19 antibacterial drugs by the disk diffusion method in accordance with the requirements of MUK 4.2.1980-04 and Clinical guidelines (2014). Data interpretation was carried out using EUCAST v. 7.0 (2017) criteria and the WHONET software package.Results. In 2016, 771 isolates of enterobacteria were isolated from the water bodies of the Ryazan region, 323 isolates from the Murmansk region. The results showed that enterobacteria were found in all surveyed surface water bodies. Citrobacter (36 %), Escherichia coli (21 %) and Providencia (21 %) dominated in the Ryazan region, while Citrobacter (35 %) and Enterobacter (21 %) dominated in the Murmansk region. Enterobacteria resistant to one or more antimicrobials dominate in both regions. The phenotype of multiple drug resistance (MDR) was found in 82.62 % of isolates in Ryazan and 95.98 % in Murmansk regions. The extreme resistance phenotype (XDR) was more common among enterobacteria isolated from water bodies of the Ryazan region. In both districts, there was a fairly high level of resistance to beta-lactam antibiotics. In both regions, the quinolones were the most effective group for inhibiting the growth of enterobacteria.Conclusion. The results of the study show that the spread of antibiotic-resistant isolates of enterobacteria in freshwater ecosystems occurs everywhere, but in northern waters this process is slower.