ANTIBIOTIC RESISTANCE OF CAUSATIVE PATHOGENES OF SURGICAL SITE INFECTION

Abstract

Microbiological monitoring is remaining a pressing issue in effective control of surgical diseases and prevention of complications. The aim of this study is to determine antibiotic resistance of causative pathogens of surgical site infections isolated in a tertiary medical setting in the Poltava region, Ukraine. This prospective observational study involved 121 middle-aged patients who underwent surgical operations in the surgical department of the Municipal Enterprise “M.V. Sklifosovsky Poltava Regional Clinical Hospital” (Poltava, Ukraine) for 2017-2020. Laboratory investigations of the biological material taken from the patients were conducted at the bacteriological department of the hospital. Pathogens were isolated and identified by an automatised technique, using Vitek2 (Biomérieux, France). 164 isolated and identified strains were determined to have the susceptibility to 30 antibiotics of various groups determined by the disk-diffusion method using standard disks with antibiotics placed on the surface of the Mueller-Hinton culture medium (HiMedia Laboratories Pvt Ltd. India). Statistical analysis was carried out: microorganisms’ susceptibility to antibiotics was calculated in percent. The highest level of resistance to antibacterial drugs was found in Pseudomonas aeruginosa, which, with the exception of 33.3% of strains were susceptible to levofloxacin only, showed resistance to all tested antibiotics. The maximum level of methicillin-resistant strains was found among coagulase-negative staphylococci and amounted to 91.3%. All isolated Klebsiella strains and 18.7% of E. coli strains were producers of extended spectrum beta-lactamases. A significant predominance of multiresistant strains of microorganisms, the resistance of which goes beyond the boundaries of more than two pharmacological groups of antibiotics, has been revealed.