Antibacterial properties and mechanisms of anti-lipopolysaccharide factor 1 from Procambarus clarkii.

Stress and disease are critical factors hindering the industrial development of red swamp crayfish (Procambarus clarkii). Breeding crayfish with stress- and disease-resistant characteristics can overcome these limitations and promote their industrial development. In this study, the crayfish breed F3, which exhibits rapid growth and a favoured haplotype combination of the immune genes R, ALF, and crustin2, encoding the Toll-like receptor, anti-lipopolysaccharide factor, and antimicrobial peptide, respectively, were selected as parents to breed offspring (the selected group (SG)). The genotype, stress resistance, and disease resistance of crayfish in the SG and unselected group (USG) were compared. The results showed that the ratio of the favoured haplotype was higher in the SG crayfish than in the USG crayfish, leading to stronger stress and disease resistance. Compared to that of the USG crayfish, the mortality of the SG crayfish subjected to stress during eight days of transport and challenged with bacteria (Aeromonas hydrophila or Vibrio parahaemolyticus) or white spot syndrome virus were significantly reduced by 60% and 20%, respectively (p < 0.05). Based on these results, the stress- and disease-resistant SG crayfish were named the “Huachizhen-1” breed. Additionally, the ratio of the unfavoured homozygous genotypes of R, ALF, and crustin2 sharply decreased, whereas those of the heterozygous genotypes increased together with stress and disease resistance during crayfish maturation under natural conditions, indicating that the heterozygotes of these genes also exhibit strong stress and disease resistance. All of this taken together, the crayfish breed “Huachizhen-1” may be applicable for improving stress and disease resistance and the production of crayfish.

An anti-lipopolysaccharide factor from red swamp crayfish, Procambarus clarkii, exhibited antimicrobial activities in vitro and in vivo

A crayfish ALF inhibits the proliferation of microbiota by binding to RPS4 and MscL of E. coli

Identification and molecular characterization of a novel anti-lipopolysaccharide factor (ALF) from red swamp crayfish, Procambarus clarkii.

The feeding of dietary Codonopsis pilosula polysaccharide enhances the immune responses, the expression of immune-related genes and the growth performance of red swamp crayfish (Procambarus clarkii)

A lysin MOTIF (LysM)-containing protein functions in antibacterial responses of red swamp crayfish, Procambarus clarkii

A novel vertebrates Toll-like receptor counterpart regulating the anti-microbial peptides expression in the freshwater crayfish, Procambarus clarkii

Effects of dietary quercetin on the innate immune response and resistance to white spot syndrome virus in Procambarusclarkii

A Lysin motif (LysM)-containing protein functions in antibacterial responses of red swamp crayfish, Procambarus clarkii

Aeromonas hydrophila associated with red spot disease in Macrobrachium nipponense and host immune-related gene expression profiles

SpALF4: A newly identified anti-lipopolysaccharide factor from the mud crab Scylla paramamosain with broad spectrum antimicrobial activity

Tumor necrosis factor alpha-induced protein 8-like mediates immune responses of Procambarus clarkii through protein-protein interactions.

A big anti-lipopolysaccharide factor showing sexual dimorphism is involved in anti-WSSV immunity in crayfish.

Chitinase involved in immune regulation by mediated the toll pathway of crustacea Procambarus clarkii

The related immunity responses of red swamp crayfish (Procambarus clarkii) following infection with Aeromonas veronii