Abstract
BackgroundRecinodindron heudelotii (Euphorbiaceae) is a plant used in Africa, particularly in Cameroon to treat various ailments including bacterial infections. In this study, we evaluated the extracts of the leaves (RHL) and bark (RHB) of R. heudelotii for their antibacterial and antibiotic resistance modulating activities against 29 Gram-negative bacteria, including multidrug-resistant (MDR) phenotypes.MethodsThe broth micro-dilution assay was used to evaluate the antibacterial activity, and the antibiotic resistance modulating effects of the plant extracts.ResultsRHL displayed the most important spectrum of activity with minimal inhibitory concentrations (MICs) values ranging from 256 to 1024 μg/mL against 75.86% of the 29 tested bacteria strains while RHB was not active. RHL also showed killing effects with minimal bactericidal concentrations (MBCs) ranging from 256 to 1024 μg/mL. The activities of tetracycline and kanamycin associated with RHL were improved on 88.89% and 77.78% of the tested MDR bacteria, at MIC/2 at MIC/4 respectively, with 2 to 16-folds decreasing of MIC. This suggests the antibiotic resistance modulating effects of these antibiotics.ConclusionThe present study provides data indicating a possible use of the leaves extract of Recinodindron heudelotii alone or in association with common antibiotics in the fight against bacterial infections including those involving MDR bacteria.
Highlights
Recinodindron heudelotii (Euphorbiaceae) is a plant used in Africa, in Cameroon to treat various ailments including bacterial infections
In the continuous search for antibacterial agents from that plant, we have designed this study to investigate the in vitro antibacterial and antibiotic resistance modulating activities of the methanol extracts from leaves and stem bark of R. heudelotii against MDR Gram-negative bacteria
Leaves extract of R. heudelotii (RHL) displayed the most important spectrum of activity with minimal inhibitory concentration (MIC) ranging from 256 to 1024 μg/mL against 75.86% of the tested bacteria while its bark extract (RHB) was active only on tree bacterial strains
Summary
Recinodindron heudelotii (Euphorbiaceae) is a plant used in Africa, in Cameroon to treat various ailments including bacterial infections. We evaluated the extracts of the leaves (RHL) and bark (RHB) of R. heudelotii for their antibacterial and antibiotic resistance modulating activities against 29 Gram-negative bacteria, including multidrug-resistant (MDR) phenotypes. Infectious diseases caused by multidrug-resistant (MDR) bacteria constitute nowadays a real public health concern all over the world. These bacteria drastically reduced the efficacy of antibiotic arsenal, increasing the frequency of therapeutic failure and mortality [1, 2]. In the continuous search for antibacterial agents from that plant, we have designed this study to investigate the in vitro antibacterial and antibiotic resistance modulating activities of the methanol extracts from leaves and stem bark of R. heudelotii against MDR Gram-negative bacteria
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