Abstract
Actinomycetes SAE4034 isolates was isolated from Rhizophora apiculata rhizosphere mud showed some antibacterial properties. The antibacterial ability of this isolate has not been tested on antibiotic resistant bacterial pathogens. However, there was no research has been reported regarding actinomycetes from Segara Anakan mangrove area resulting compounds inhibit the growth of antibiotics-resistant bacteria. Therefore, it is important to investigate its capability against antibiotics resistant bacteria or multi drug resistant bacteria (MDR bacteria). The research aimed to know the ability of actinomycetes SAE4034 in inhibit MDR bacteria and to identify the species profiles. The research methods included isolate characterization involving morphology, physiology/enzymatic and molecular properties. MDR bacterial inhibition assay, antibacterial compound extraction and antibacterial compound test using thin layer chromatography (TLC) method, observation of morphological and biochemical properties, DNA isolation, amplification and analysis of 16SrRNA sequence, and phylogeny tree analysis. The methods of this study included MDR anti-bacterial assay and antibacterial compound test. Subsequent step was isolate characterization including observation of morphological and physiological / enzymatic properties, and 16S rRNA gene sequence. The results showed that culture extract was able to inhibit the growth of MDR bacteria i.e. Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus sp., but no inhibition to Enterobacter cloacae. The bioactive compound showed 4 spots with Rf values of 0.36; 0.45; 0.54; and 0.6. Based on morphology, physiology / enzymatic and 16S rRNA gene sequences characteristics, actinomycetes SAE4034 isolate is Streptomyces sp. This research showed new Streptomyces strain that serves as a source of MDR antibacterial compounds and useful in development of antibiotic for combating infectious diseases caused by MDR bacteria
Highlights
Actinomycetes is prokaryotic which most plays a role in producing secondary metabolites, including antibiotics (Bhat et al, 2013)
Actinomycetes SAE4034 broth culture grown in Starch Casein Broth (SCB) and Starch Nitrate Broth (SNB) media have different inhibitory capabilities against MDR bacteria
SCB culture filtrate showed inhibition to S. aureus (φ 9 mm) and no inhibition showed to other MDR bacterial growth
Summary
Actinomycetes is prokaryotic which most plays a role in producing secondary metabolites, including antibiotics (Bhat et al, 2013). Various types of antibiotics produced by actinomycetes include aminoglycosides, anthracyclines, glycopeptides, beta-lactams, macrolides, nucleosides, peptides, polyene, polypeptides, actinomycin, and tetracyclines (Kekuda et al, 2010; Bhat et al, 2013). Most antibiotics are produced by Streptomyces and Micromonospores (Bhat et al, 2013). Streptomyces is an unrivaled species in producing secondary metabolite natural products (Radjasa, 2013). Sequence 16S is a sequence of genes in the prokaryotes that have conserved nucleotide sequences, so it can be used as a guide to compare the identity of actinomycetes species
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biosaintifika: Journal of Biology & Biology Education
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
