Abstract
Background Antimicrobial drug resistance is one of the serious issues this world is facing nowadays, and increased cost of searching for effective antimicrobial agents and the decreased rate of new drug discovery have made the situation increasingly worrisome. Objective The aim of this study is to determine in vitro antibacterial activity of honey against methicillin-resistant Staphylococcus aureus isolates from wound infection. Methods An experimental study was conducted from May to November 2017. Methicillin resistance was detected using cefoxitin (30 μg) and oxacillin (1 μg) antibiotic discs. Different concentrations of honey (25–100% v/v) were tested against each type of clinical isolates obtained from wound infection. A preliminary sensitivity test was done to all types of honey by using disk diffusion while minimum inhibitory concentration and minimum bactericidal concentration were determined for the most potent honey by the broth dilution technique. All statistical analysis was performed by using Statistical Package for the Social Sciences version 20. Results In this study, 36 bacterial isolates were recovered from 50 specimens, showing an isolation rate of 72%. The predominant bacteria isolated from the infected wounds were Staphylococcus aureus (15, 41.7%). Among identified Staphylococcus aureus, methicillin resistance accounts for 10 isolates (27.8%). All isolates showed a high frequency of resistance to tetracycline. Four collected honey varieties exhibited antibacterial activity, while the strongest inhibitory activity was demonstrated by honey-2 at 75% v/v. The mean MIC and MBC of honey-2 ranged from 9.38 to 37.5% v/v. Conclusions Tested honey has both a bacteriostatic and bactericidal activity. Among the tested honey, “honey-2” had high antibacterial potency than others.
Highlights
Inadequate wound management compounded with secondary infection is still remaining a major public health problem in developing countries. erefore, wound management has remained as research focus so far
Anti-infective drugs are critically important in reducing the global burden of infectious diseases [8, 9]. e occurrence of drug-resistant microorganisms diminished the development of antibiotics, and few pharmaceutical companies remain active in this area, posing a big challenge in this world [10]
The purpose of this study was to evaluate antibacterial activity of honey against Methicillin-resistant Staphylococcus aureus (MRSA) isolates from an infected wound in Gamo Gofa Zone, South Ethiopia, so that they would have been recommended as therapeutic agents after pharmaceutical standardization and clinical trials
Summary
Inadequate wound management compounded with secondary infection is still remaining a major public health problem in developing countries. erefore, wound management has remained as research focus so far. Inadequate wound management compounded with secondary infection is still remaining a major public health problem in developing countries. E occurrence of drug-resistant microorganisms diminished the development of antibiotics, and few pharmaceutical companies remain active in this area, posing a big challenge in this world [10]. E aim of this study is to determine in vitro antibacterial activity of honey against methicillin-resistant Staphylococcus aureus isolates from wound infection. Different concentrations of honey (25–100% v/v) were tested against each type of clinical isolates obtained from wound infection. E predominant bacteria isolated from the infected wounds were Staphylococcus aureus (15, 41.7%). Among identified Staphylococcus aureus, methicillin resistance accounts for 10 isolates (27.8%). Tested honey has both a bacteriostatic and bactericidal activity. “honey-2” had high antibacterial potency than others
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