Abstract
Escherichia coli is a gram-negative bacteria known to cause diarrhea and infection and is frequently found in foodstuffs. These bacteria may be susceptible to the microbial activity of kingkit orange due to the presence of active compounds, such as flavonoids, tannins, terpenoids, and alkaloids. Antibiotics are commonly used to treat infections, but inappropriate dose levels can cause resistance. Therefore, this study aims to determine the antibacterial effectiveness of kingkit orange fruit and leaf extracts against E. coli infection by in vitro. The materials used included fruit and leaf extract of the kingkit oranges with five concentration levels, i.e., 5%, 10%, 15%, 20%, and 25%. Observational data were analyzed using the complete randomized block design method and the least significant different follow-up test at the 5% level. The best concentration of kingkit orange fruit extract to reduce the growth of E. coli was 25%, and the diameter of the inhibition area was 6.65 mm with moderate inhibition strength. The best concentrations of Kingkit orange leaf extract to reduce the growth of E. coli were 20% and 25% with diameters of inhibition areas of 6.82 mm and 7.15 mm, respectively, with medium inhibition strength. It can be concluded that extracts from kingkit orange fruit and leaf have antibacterial activity, a potential substitute for antibiotics.
Published Version
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