Abstract
BackgroundThe current study was to evaluate the anti-thrombotic effect of alpha-linolenic acid (ALA) which was isolated and purified from Jiaomu in vivo.MethodsThe seeds were crushed and subsequently subjected to saponification, acid hydrolysis, gradient freezing, urea inclusion and complexation of silver nitrate to obtain the unsaturated fatty acids. The chemical characteristics of isolated ALA were validated by 1HNMR, 13CNMR and mass spectrometry, and then the anti-thrombotic effect of ALA and its mixture with linoleic acid (1:1) were evaluated in the following experiments.ResultsThe alpha-linolenic acid was isolated and purified from Jiaomu through our newly established methods. ALA and its mixture with linoleic acid can prolong the hemorrhage and coagulation time as well as enhanced the survival rate of mice subjected to collagen-adrenaline induced thrombosis. In addition, the thrombosis on A-V bypass and platelet aggregation of rats will be reduced after treated with ALA or its mixture, and the expression level of Akt and PI3K protein decreased 26% and 31%, respectively.ConclusionsWe designed and optimized a very simple and high-yield procedure to isolate ALA and linoleic acid mixture from seeds of Zanthoxylum bungeanum Maxim and demonstrated that such mixture can obtain a good anti-thrombotic effect through the modulation of PI3K/Akt signaling.
Highlights
The current study was to evaluate the anti-thrombotic effect of alpha-linolenic acid (ALA) which was isolated and purified from Jiaomu in vivo
Similar to other essential fatty acids, ALA is critical for human bodily function given its conversion into the longer chain fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)
Chemical characteristics The characteristics of isolated and purified alpha-linolenic acid (ALA) from Jiaomu were evaluated through the following experiments
Summary
The current study was to evaluate the anti-thrombotic effect of alpha-linolenic acid (ALA) which was isolated and purified from Jiaomu in vivo. Alpha-linolenic acid (ALA), which belongs to a group of fatty acids named omega-3 fatty acids, is an essential fatty acid. Seed oils are the richest sources of ALA, especially those of rapeseed, soybeans, walnuts, flaxseed, perilla, chia and hemp. ALA can be obtained from thylakoid membranes of the green leaves of broadleaf plants [1,2]. Similar to other essential fatty acids, ALA is critical for human bodily function given its conversion into the longer chain fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). It was reported that ALA may possess the anti-arrhythmic properties after
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