Abstract
The antiphytopathogenic effects of the crude methanol extract of Cladophora glomerata (Lin.) Kützing (Cladophoraceae) which isolated from Al Rashidiya region at north of Baghdad was investigated against two type of plant fungi (Pythium altimum and Rhizoctonia solani) which causes damping off disease where isolated from covered cucumber field in Al-Alyosifia region. Hot methanol extract showed antifungal activity against the two species of fungi in different concentrations(10,25,50 mg/ml) of extract to Cladophora glomerata as percentage inhibition (51.63 ,72.8 ,83.71) and (56.18 ,77.41 ,100)comparing with Pythium altimum and Rhizoctonia solani respectively .primary detection of active compounds showed that macroalgae (Cladophora glomerata) containing flavonoids,alkaloids, phenols and tannins. Our findings suggest the possibility of using the Cladophora glomerata as a novel source of natural antimicrobial agents for pharmaceutical industries.
Highlights
Algae are a large and diverse group of simple, typically autotrophic organisms, ranging from unicellular to multicellular forms and fall under the category macro-algae
Antifungal Activity Assay: In this experiment the crude extracts of the macro algae were mixed with Potato Dextrose Agar (PDA) medium to get different concentrations (50,25,10 ) mg/ml and the fungal mycelia were inoculated to grow
Vol.11(2)2014 between (51.63%-83.71%) and (56.18%-100%) to Pythium ultimum and Rhizoctonia solani respectively at different concentrations of extract
Summary
Algae are a large and diverse group of simple, typically autotrophic organisms, ranging from unicellular to multicellular forms and fall under the category macro-algae. Micro-algae are, as the name suggests, microscopic photosynthetic organisms. Like macro algae, these organisms grow very rapidly, and are found in both marine and fresh water environment[1]. Researchers found that algae contain remakeable amount of components valuable for human health. Amongst algae, those have been reported to inhibit growth of micro organisms are mostly planktonic and benthic fresh and marine water [2]. Many marine macro algae produce a variety of secondary metabolites [3]. These metabolites are mainly terpenes, alkaloids and polyphenolics, many of these compounds being halogenated [4]. Glomerata grows attached to hard substrates in nutrient rich alkaline freshwaters, high morphological plasticity makes classification to species level difficult[8]
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