Abstract
IT is generally accepted that the penicilloyl group is the major determinant in penicillin allergy1,2. It is probably formed in vivo either by the coupling reaction between the penicillin molecule and proteins3,4, or by way of penicillenic acid5. Various in vitro and in vivo methods, including passive haemagglutination, skin test, as well as passive transfer1,2, have been used for the detection of anti-penicilloyl antibodies. Because the detection of antibodies depends on the sensitivity of the assay used, development of more sensitive techniques seemed worth while, and fluorescence polarization6 and a sensitive passive haemagglutination method2 for detection of anti-penicillin antibodies were recently described. A very sensitive assay for the determination of extremely low concentrations of antibodies to defined determinants, making use of haptens coupled chemically to bacteriophage, has been reported recently by Makela7 as well as by Haimovich and Sela8. We have now extended this method to the detection and quantitative determination of anti-penicilloyl antibodies.
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