Abstract
Anti-aging effects of the proteins from artemia extract on human fibroblasts cell proliferation and collagen expression in induced aging conditions
Highlights
Aging and senescence are characterized by the reduction of response to stress, increasing homeostatic imbalance and increased risk of diseases [1]
Artemia extract treated fibroblasts were collected and total RNA was extracted by using the tripure isolation reagent (Roche, Mannheim, Germany), according to the manufactures protocol
First-strand cDNA was synthesized from 1 μg of total RNA using Maurine Maloney Leukemia virus (M-MLV, Fermentase) and reverse transcriptase
Summary
Aging and senescence are characterized by the reduction of response to stress, increasing homeostatic imbalance and increased risk of diseases [1] Many factors such as genetic elements, free radicals, oxidants, Reactive Oxygen Species (ROS), telomeres shortening, alcohol and etc. Normal somatic cells invariably enter a state of irreversibly arrested growth and altered function after a finite number of divisions [5]. Evidences indicated that, this process is related to a tumor-suppressive mechanism and an underlying cause of aging [6]. In hemostasis some anti-aging factors, such as ascorbate, tocopherol and carotenoids neutralize aging inducing factors
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