Abstract
Anthocyanins composition of five purple leaves cultivars of Ocimum basilicum L. was investigated by reversed-phase HPLC with mass-spectrometric detection in the conditions of ions partial fragmentation as well as preparation of dried differently colored forms of anthocyanins encapsulated into maltodextrin matrix. Mass-spectra analysis revealed that according to anthocyanin set basil cultivars under investigation may be divided onto two groups with common feature being high level of acylation with (mainly) p-coumaric, ferulic and malonic acids of the same base – cyanidin-3-dihexoside-5-hexoside. The presence of acylation with substituted cinnamic acids permits to obtain solutions not only with red color (the property of the flavylium form) but also with blue shades coloration due to quinoid and negatively charged quinoid forms. All forms exept of flavylium are not stable in solution but stable enough to prepare dried encapsulated forms by lyophilization. And thou the loss of anthocyanins at drying is not negligible the final product is characterized with high stability at storage in refrigerator.
Highlights
Род базилик (Ocimum) относится к подсемейству котовниковые (Nepetoideae) семейства яснотковые (Lamiaceae)
Anthocyanins composition of five purple leaves cultivars of Ocimum basilicum L. was investigated by reversed-phase HPLC with mass-spectrometric detection in the conditions of ions partial fragmentation as well as preparation of dried differently colored forms of anthocyanins encapsulated into maltodextrin matrix
Mass-spectra analysis revealed that according to anthocyanin set basil cultivars under investigation may be divided onto two groups with common feature being high level of acylation with (mainly) p-coumaric, ferulic and malonic acids of the same base – cyanidin-3-dihexoside-5-hexoside
Summary
Растительный материал выращивали в открытом грунте на приусадебном участке в сезоне 2017 г., используя рассадный метод. Антоцианы экстрагировали из листьев растений настаиванием в 0,1 М водном растворе HCl. Экстракт очищали методом твердофазной экстракции [9]. Перед получением инкапсулированных форм концентрацию антоцианов определяли в 0,1 М водном растворе HCl упрощенным спектрофотометрическим методом (без вычитания абсорбции при рН = 4,5), используя коэффициент экстинкции 26900 л∙моль-1∙см-1 для пересчета на цианидин-3-глюкозид хлорид [10]. Для этого к раствору антоциана с определенным перед изменением рН содержанием и суммы антоцианов добавляли расчетное количество мальтодекстрина. Для определения антоцианов методом ВЭЖХ использовали хроматографическую систему Agilent 1260 Infinity с диодно-матричным и масс-спектрометрическим (Agilent 6130 Quadrupole LC/MS) детекторами. Масс-спектры записывали в режиме ESI (ионизация распылением в электрическом поле) со сканированием положительных ионов; напряжение на фрагменторе – 300 В. Хроматограмму записывали при 515 нм, хранили и обрабатывали, используя программу ChemStation 32
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