Annona muricata (Soursop) Leaves Extract Increased Caspase 3 Expression in Patient-derived WHO III Nasopharyngeal Cancer Cell

Abstract

Event Abstract Back to Event Annona muricata (Soursop) leaves extract increased caspase 3 expression in patient-derived WHO III nasopharyngeal cancer cell Ahmad D. Wahyudiono1*, Ersty Istyawati1 and Pudji Rahaju1 1 Department of Otorhinolaryngology, University of Brawijaya, Indonesia Background Nasopharyngeal cancer is the most common head and neck cancer in Indonesia. Annona muricata leaves extract contains annonaceous acetogenin, which have emerged as potentially promising anti cancer drugs. The purpose of this study is to investigate the effect of soursoup (Annona muricata) leaves fraction in inducing caspase 3 expression in cell cultured from biopsy tissue of nasopharyngeal cancer WHO III. Methods This study is a true experimental study that use post test only control group design. This study uses cell culture and divided into 5 group treatments. Soursop leaves extraction uses ethanol 95%. Bioactive agents used are fraction materials from crude extract using hexane and methanol. There are 5 group dosage 0, 31.25 µg/mL, 62.5 µg/mL, 125 µg/mL dan 250 µg/mL. The caspase 3 expression was detected by caspase 3 elisa assay kit observed in 6, 12 and 24 hours. Results Expression of caspase 3 in treatment group is higher than control group. The expression increased by time and the highest is in 24 hours after treatment. Increased in dosage was also followed by increased in caspase 3 expression especially at doses of 125 µg/mL and 250 µg/ml. Conclusion Annona muricata leaves extract induced caspase 3 expression in WHO III nasopharyngeal cancer cultured cells Keywords: Annona muricata, Caspase 3 expression, nasopharyngeal cancer WHO III, Acetogenin, leaves fraction Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Cancer Citation: Wahyudiono AD, Istyawati E and Rahaju P (2019). Annona muricata (Soursop) leaves extract increased caspase 3 expression in patient-derived WHO III nasopharyngeal cancer cell. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00020 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 05 Nov 2018; Published Online: 17 Jan 2019. * Correspondence: MD. Ahmad D Wahyudiono, Department of Otorhinolaryngology, University of Brawijaya, Malang, Indonesia, dian.punk7@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ahmad D Wahyudiono Ersty Istyawati Pudji Rahaju Google Ahmad D Wahyudiono Ersty Istyawati Pudji Rahaju Google Scholar Ahmad D Wahyudiono Ersty Istyawati Pudji Rahaju PubMed Ahmad D Wahyudiono Ersty Istyawati Pudji Rahaju Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.