Abstract

Negative ion thermospray (TSP) tandem mass spectrometry (MS/MS) was used to determine phenols and their corresponding glucuronide and sulfate conjugates. Nine model compounds were separated to baseline on a strong anion exchange (SAX) LC column eluted with an ammonium formate buffer-acetonitrile mobile phase. Selected reaction monitoring in LC/MS/MS allowed glucuronic- and sulfate-selective detection as well as specific detection of xenobiotics and their elaborated conjugates

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