Abstract
An ultra pressure liquid chromatography (UPLC)/MS/MS method for vancomycin and teicoplanin determination in human plasma was developed in accordance with analytical quality by design (AQbD) concept and fully validated. Chromatographic separation was performed on ACQUITY UPLC C18 charge surface hybrid (CSH) column (2.1mm×50mm, 1.7μm particle size) in gradient mode and the mobile phase consisted of 0.1% formic acid in water and pure acetonitrile. The experimental design methodology was used for the definition of optimal chromatographic and protein precipitation conditions. The linearity ranges were 0.05-10μg ml-1 for vancomycin and 0.5-200μg ml-1 for total teicoplanin. The relative standard deviations for precision estimation were below 15% and the accuracy was within 85-115% for all quality control levels. The method was utilized for glycopeptide antibiotics bioanalysis.
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