Abstract
Lysozymes as an important immune factor, play vital roles in innate immune response against pathogen infection. In the present study, one c-type and g-type lysozymes were identified from Chinese giant salamander (Andrias davidianus). They shared highly conserved structural features with lysozymes from other species. Spatial expression analysis revealed that AdlysC transcript was most abundant in liver and stomach, and least in muscle and brain. In contrast, the expression level of AdlysG was most abundant in liver and least in muscle and skin. The transcription level of c-type and g-type lysozymes were up-regulated after Aeromonas hydrophila infection in liver and spleen, indicating their participations in the immune response. Moreover, the recombinant AdlysC and AdlysG protein were produced and purified, and were used to investigate the lysozyme activity at different pH and temperatures. The optimal lytic activity was determined at pH 6.0 and at a temperature of 30 °C. Through the minimal inhibitory concentration test, the rAdlysC and rAdlysG exhibited apparent antibacterial activity against both Gram-positive and Gram-negative bacteria with a variable concentration. In conclusion, it is the first report of lysozymes in A. davidianus, and c-type and g-type lysozymes should be involved in the innate immune response of A. davidianus.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
