Abstract
BackgroundThe purpose of the present study was to evaluate the correlation between Torque teno sus virus 1b (TTSuV1b) infection and other viral infections or vaccine immunization in conventional pigs.MethodsWith overexpressed and purified viral protein TTSuV1b as antigen, an indirect enzyme-linked immunosorbent assay (ELISA) method for detecting TTSuV1b antibody was established, which demonstrated great specificity and reproducibility. Porcine serum samples (n = 212) were tested using ELISA. Meanwhile, the antibodies against Classical Swine Fever Virus (CSFV), Pseudorabies Virus (PRV), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), and Porcine Circovirus 2 (PCV2) were also examined using the commercial kits.ResultsStatistical analysis indicated that the level of anti-TTSuV1b antibody was positively correlated with the level of anti-PCV2 antibody in a lesser extent; the level of antibodies against TTSuV1b or PCV2 were significantly lower in porcine serum with low level of TTSuV1b virus, implicating the potential consistency and synchronization in the mechanism of TTSuV1b and PCV2 infection. Whereas, antibodies against PRRSV or CSFV showed no statistical significance on comparison with anti-TTSuV1b antibody, implicating that in conventional pigs, the antibody level for PRRSV and CSFV were not significantly influenced by TTSuV1b infection.ConclusionIn conclusion, examination of anti-TTSuV1b antibody in porcine serum with the presently established ELISA method would serve as a supplementary approach for etiological investigation, and the combined statistical analysis of the antibodies against four other viruses might help to further understand the TTSuV1b infection as well as its pathogenicity.
Highlights
The purpose of the present study was to evaluate the correlation between Torque teno sus virus 1b (TTSuV1b) infection and other viral infections or vaccine immunization in conventional pigs
Owing to the relative high homology of TTSuV1b ORF1a to the corresponding area of previously reported TTSuV1b strains, this polypeptide should be a good representative for research of TTSuV1b antigenicity
Protein expression and purification of the recombinant TTSuV1b ORF1a The recombinant engineering of bacteria TTSuV1b ORF1a-pET21/BL21 was generated by transformation of TTSuV1b ORF1a-pET21 plasmid into E. coli BL21
Summary
The purpose of the present study was to evaluate the correlation between Torque teno sus virus 1b (TTSuV1b) infection and other viral infections or vaccine immunization in conventional pigs. Torque teno virus (TTV) is an icosahedral, circular, negative single-stranded deoxyribonucleic acid (ssDNA) virus without envelope. The diameter of this virus particle is 30–32 nm as observed under an electron microscope. According to the 9th report of the International Committee on Taxonomy of Viruses (ICTV) in 2009, Torque teno sus virus (TTSuV), the TTV which infects pigs, belongs to the family of Anelloviridae and the genus of Iotatorquevirus, and it is composed of two species: Torque teno sus virus. In 2011, a new genus Kappatorquevirus, including a species of Torque teno sus virus k2, was supplemented by ICTV; later, the TTSuV1 and TTSuV2 were renamed TTSuV1a and TTSuV1b respectively, in relation to newly added Torque teno sus virus k2 [4].
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