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Analysis of the interplay between hepatitis B virus-positive hepatocytes and Kupffer cells e x vivo using mice as a model.

Publication Date Jun 17, 2022

Abstract

Kupffer cells play critical roles in both hepatitis B virus (HBV) persistence and clearance. Here, we provide a protocol for studying the interplay between Kupffer cells and HBV-positive hepatocytes ex vivo using mice as a model. This protocol includes hydrodynamic injection of HBV DNA into mouse hepatocytes, liver perfusion for isolating hepatocytes and Kupffer cells, and Seahorse metabolic analysis of Kupffer cells. This protocol allows the detailed analysis of how HBV-positive hepatocytes and Kupffer cells impact each other ex vivo. For complete details on the use and execution of this protocol, please refer to Li etal. (2022).

Concepts

Kupffer Cells Hepatitis B Virus Mouse Hepatocytes HBV-positive Cells Virus-positive Cells Injection Of DNA Virus Clearance Virus Persistence Seahorse Metabolic Analysis Hepatitis B Virus DNA

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No potential conflict of interest was reported by the authors. The conception and design of the study, acquisition of data, analysis and interpretatio...

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