Abstract
Application of apolar glass capillary columns to steroid analysis by gas chromatography is described. Following a relatively simple clean-up procedure the isolated urinary steroids were separated in the gas phase as their methoxime trimethylsilyl derivatives. Total urinary steroid profiles are shown, being of diagnostic value in studying irregularities in steroid metabolism. Special attention is paid to the identification of the separated steroids by retention indices and methylene unit values. In more detailed studies, structure elucidation by means of a gas chromatograph—mass spectrometer combination was of invaluable help, especially when dealing with previously unknown metabolites.
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