Abstract

Introduction Fourier-transform infrared (FT-IR) spectroscopy is a technique with great potential for body fluids analyses. The aim of this study was to examine the impact of session training on cortisol concentrations in rugby players by means of infrared analysis of serum. Methods Blood collections were performed pre, post and 24 hours after of rugby training sessions. Serum cortisol was analyzed by FT-IR spectroscopy and chemiluminescent immunoassay. Results There was a significant difference between the integrated area, in the region of 1180-1102 cm-1, of the spectra for pre, post and post 24 h serums. The cortisol concentration obtained by chemiluminescent immunoassay showed no significant difference between pre, post and post 24 h. Positive correlations were obtained between the techniques (r = 0.75), post (r = 0.83) and post 24 h (r = 0.73). Conclusion The results showed no increase in cortisol levels of the players after the training sessions, as well as positive correlations indicating that FT-IR spectroscopy have produced promising results for the analysis of serum for diagnosis of stress.

Highlights

  • Fourier-transform infrared (FT-IR) spectroscopy is a technique with great potential for body fluids analyses

  • The aim of this study was to examine the impact of session training on the serum cortisol concentrations in rugby players, using FT-IR spectroscopy and chemiluminescent immunoassay method

  • The training load was used as an indicator of exercise intensity since it has been widely used as a useful tool for prescribing exercise intensity, due to its relationship with physiological indicators of exercise stress (Irving et al, 2006; Nakamura et al, 2010)

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Summary

Introduction

Fourier-transform infrared (FT-IR) spectroscopy is a technique with great potential for body fluids analyses. Conclusion: The results showed no increase in cortisol levels of the players after the training sessions, as well as positive correlations indicating that FT-IR spectroscopy have produced promising results for the analysis of serum for diagnosis of stress. A successful process requires the interaction between the training load and recovery time, in order to avoid stress, which can lead to decreased performance and increased risk of injury (Ferrari et al, 2013; Meeusen et al, 2013). This imbalance between the overall strain experienced during exercise training and the athlete’s tolerance to such effort may induce overreaching or overtraining syndrome, which are characterized by diminished sport-specific physical performance, accelerated fatiguability and subjective symptoms of stress (Urhausen et al, 1995).

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