Abstract
BackgroundAnopheles culicifacies sensu lato is an important vector of malaria in Southeast Asia contributing to almost 70% of malaria cases in India. It exists as morphologically similar sibling species A, B, C, D and E with varied geographical distribution patterns. Vector control measures have been difficult for this important vector as the sibling species have developed varying levels of resistance to the currently used insecticides. In view of the importance of this vector, we developed and validated a set of microsatellite markers and the same were used to analyze the population genetic structure of five different geographical populations of An. culicifacies A.MethodsAnopheles culicifacies A samples were collected from different localities across India, and genotyping was performed using eight microsatellite markers on ABI Prism 310 Genetic Analyzer. Several statistical analyses were performed to ascertain the genetic diversity that exists within and between the populations.ResultsThe markers were found to be moderately polymorphic in the populations. Genetic analysis indicated significant genetic differentiation between the majority of the population pairs analyzed and was not found to be related to the geographical distances between populations.ConclusionThis is the first and successful attempt to test the microsatellite markers developed for population genetic analysis of An. culicifacies A. Host feeding and breeding habits of species A suggest that factors other than ecological and geographical barriers were responsible for the genetic differentiation that has been observed between the populations.
Highlights
Anopheles culicifacies sensu lato is an important vector of malaria in Southeast Asia contributing to almost 70% of malaria cases in India
Two major vectors found in India, An. stephensi an urban malaria vector [11] and An. baimaii, a vector in north eastern states [12] were analysed for population genetic structure and gene flow using microsatellite markers and mitochondrial DNA genes respectively
Realizing the importance of knowledge on the population structure and gene flow for implementing insecticide resistance management strategies for the control of An. culicifacies sibling species, microsatellite markers developed in our laboratory [13] were used in this study to understand the population structure of An. culicifacies species A populations, and the results are reported in this paper
Summary
Anopheles culicifacies sensu lato is an important vector of malaria in Southeast Asia contributing to almost 70% of malaria cases in India. Anopheles culicifacies sensu lato has a wide distribution in India and extends into the west to Ethiopia, Yemen, Iran, Afghanistan and Pakistan, and in the east to Bangladesh, Myanmar, Thailand, Cambodia and Vietnam and is found in Nepal and southern China in the north and extends to Sri Lanka in the south [1,2]. It is an important malaria vector in India, Sri Lanka and in the countries west of India. Realizing the importance of knowledge on the population structure and gene flow for implementing insecticide resistance management strategies for the control of An. culicifacies sibling species, microsatellite markers developed in our laboratory [13] were used in this study to understand the population structure of An. culicifacies species A populations, and the results are reported in this paper
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
