Abstract
A sensitive method for the quantitative analysis of putrescine, spermidine, and spermine is described. Tissue extracts prepared with cold 5% trichloroacetic acid are freed of the acid by ether extraction and purified by either n-butanol extraction or a rapid cation-exchange column procedure. The n-butanol extracts or HCl eluates from the column containing the tissue putrescine, spermidine, and spermine are concentrated and chromatographed on thin-layer plates of Whatman cellulose CC41. The amines are reacted with ninhydrin and the colored thin-layer spots are scraped from the plate and extracted and the absorbance is measured at 575 mμ. The method is precise and is adapted to the analysis of polyamines in diverse biological materials.
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