Abstract
A method for determination of ochratoxin A (OTA) in wines using a new-solid phase extraction clean-up procedure followed with ultra performance liquid chromatography (UHPLC)-Orbitrap MS based on two scan events (full-scan Fourier transform mass spectrometer [FTMS] and higher energy-induced collision dissociation[HCD] data-dependent MS/MS) in positive ionization mode has been developed. The limit of detection (LOD) was estimated at 0.46 μg l−1 for white wine, 0.53 and 0.54 μg l−1 for rose and red wines, respectively. The limit of quantification (LOQ) was estimated at 1.57 μg l−1 in white wine, 1.77 and 1.81 μg l−1 in rose and red wines. Recovery experiments were carried out with spiked samples at three concentration levels (2, 5 and 10 μg l−1). The OTA recoveries in spiked white wine samples varied from 69.6 % to 99.8 %, while the recoveries for rose and red wine samples were in the range of 63.0–110.2 % and 63.6–103.2 %, respectively. Finally, based on the results, it is concluded that the combination of C18 cartridge with conventional particle packed columns and UHPLC LTQ-Orbitrap XL is an appropriate procedure for OTA analysis in wines.
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