Analysis of human papillomavirus infection in non-small cell lung cancer

Abstract

Objective To detect the infection of human papillomavirus (HPV) in patients with non-small cell lung cancer (NSCLC) and explore the relationship between HPV infection and clinicopathological features. Methods HPV detection and genotyping in 156 cases of NSCLC were performed using a new liquid chip based on Luminex. Patient clinical characteristics were also recorded, and the relationship between HPV infection and clinicopathological features was studied. Results Of the 156 tumor-DNA samples tested, 40 (25.6%) cases showed presence of HPV-DNA, of which 37 cases were of a high-risk HPV type (16, 18, 33, 58). The differences were statistically significant between the HPV-positive and HPV-negative groups in gender (χ2=4.387, P=0.036), smoking (χ2=8.130, P=0.004), histologic type (χ2=4.075, P=0.044) and lymph node metastasis (χ2=7.082, P=0.008). The differences were not statistically significant between the HPV-positive and HPV-negative groups in age (χ2=0.013, P=0.910), differentiated degree (χ2=1.727, P=0.189), clinical stages (χ2=0.179, P=0.672), distant metastasis (χ2=3.012, P=0.083). Logistic regression analysis revealed that lymph node metastasis alone was an independent predictive factor of HPV infection in NSCLC (OR=0.384, 95%CI: 0.153-0.967, P=0.042), and gender (OR=1.402, 95%CI: 0.522-3.769, P=0.503), smoking (OR=0.506, 95%CI: 0.194-1.322, P=0.506), histologic type (OR=0.393, 95%CI: 0.133-0.161, P=0.091) were not independent predictive factors of HPV infection. Conclusion The infection of HPV presents in part of Chinese NSCLC patients, and HPV infection may be connected with occurrence and development of lung cancer. Key words: Carcinoma, non-small cell lung cancer; Human, papillomavirus; Luminex technology