Abstract
Previously we reported the identification of seven quantitative trait loci (QTLs) associated with the rice yield measuring five parameters including panicles per plant (PPP), spikelets per panicle (SPP), seed set percentage (SSP), 1000-grain weight (TGW) and yield in 2012. Here we report the analysis of QTLs using the same trait parameters data of the mapping population in 2013 for detecting highly conserved QTL markers. A total of 6 QTLs were identified from chromosomes 1, 7, 8, 10, 11, and 12, which were contrasted with our previous results (chromosomes 1, 2, 4, 5, 6, 8, and 11). In this comparison, three QTLs from chromosome 1, 8, and 11 were only found to be associated with the components of yield over two consecutive years indicating high sensitivity of QTL markers to the environment. Of those three QTLs, SPP-associated marker RM12285 was found to be dominantly expressed by real-time PCR (qPCR). In addition, compared to our previous report the numbers of mapping population and markers were significantly increased for higher resolution markers from 70 to 120, and from 143 to 217, respectively. We also found that the parameter SPP was dominantly correlated with the rice yield. Furthermore, the double haploid (DH) population facilitated to analyze the epistatic effects for yield and yield components in rice. Taken together, combining multiple mapping population data over years possibly enables narrowing down to the highly conserved QTL markers against diverse environmental fluctuation caused by such as drought and high temperature. Thus, these data would be critically exploited to improve for the crop breeding strategy.
Highlights
One of the most demanded crops is rice being favored by more than half of the world population
Phenotypic transgressive and continuous variations were observed for most traits. 2012 “Nagdong” had higher values for spikelets per panicle, seed set percentage and grain yield, while 2012 “Cheongcheong” had higher panicles per plant and 1000-grain weight. 2013 “Nagdong” had higher values for seed set percentage. 2013 “Cheongcheong” had higher panicles per plant, spikelets per panicle, and grain yield
Negative correlations existed among panicles per plant, spikelets per panicle and 1000-grain weight (Table 2)
Summary
One of the most demanded crops is rice being favored by more than half of the world population. Even though the development of new varieties by crop breeding program has been operated successfully, the significant increment of world population hardly attenuates the problem of famine, in developing countries. For this reason, it is still a top priority to increase the yield of rice. Further to our previous report that investigated the QTLs associated with yield in a single year-produced mapping population, in this study we collected a second consecutive year-produced data to improve selective efficiency via detection on QTLs controlling yield and yield components in different environments. More conserved QTL markers with higher credibility under diverse environmental conditions might significantly elevate the efficiency of breeding strategy for the development of new crop varieties
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