Abstract
Lipid peroxidation produces many primary and secondary oxidation products such as malondialdehyde (MDA) which have been widely used in the evaluation of oxidative status in various food and biological samples. The presence of MDA in oil samples, not only could decrease its quality and nutritional characteristics but also represents a potential risk to human health, due to its high reactivity with proteins and nucleic acids. A new analytical methodology is proposed for the assessment of MDA in oils using a gas-diffusion microextraction (GDME) with thiobarbituric acid (TBA) derivatization procedure followed by high-performance liquid chromatography with ultraviolet and fluorescence detection (HPLC-UV-FLD) analysis. This procedure was developed and validated presenting good linearity (r2≥0.9947) in the range of 0.8–10 and 1–10 μg g−1 for UV and FLD, respectively. Precision was assessed, showing excellent results. The methodology was applied for the first time to the determination of MDA in fifty-four edible oils. Hierarchical cluster analysis (HCA) was used to correlate lipid peroxidation with oil processing.
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