Abstract
This article studies the sterilization effects of high-voltage pulsed electric field (PEF) of technology on filamentous fungi. A cell dielectric model was proposed based on the physical structure of filamentous fungi. Basic theories of the electromagnetic field were comprehensively applied, and the multiphysics field simulation software COMSOL Multiphysics was used for more detailed study. The effects of PEF treatment parameters and microbial characteristic parameters on the resulting cell membrane and nuclear membrane changes were simulated and analyzed. The results showed significant effects on the transmembrane voltage of the cell membrane and nuclear membrane from the electric field intensity, pulse duration, cell membrane thickness, superposition effect of the pulses. However, the amount of hyphae had little effect, and the number of cell nuclei and the thickness of the cell walls had almost no effect on the transmembrane voltage of the cell membranes and the nuclear membranes. The results provide theoretical support for applying high-voltage PEFs to kill fungi in practical applications.
Highlights
Pulsed Electric Field (PEF) sterilization technology is an emerging and popular sterilization method in recent years [1]
This paper describes research on the effects found for how the parameters of high-voltage PEF treatment effect the inner and outer membrane transmembrane voltage of filamentous fungi
The electric field intensity has a significant impact on the nuclear membrane transmembrane voltage
Summary
Pulsed Electric Field (PEF) sterilization technology is an emerging and popular sterilization method in recent years [1]. Its characteristics include low processing temperature, short processing time and low energy consumption. Take into account these characteristics, it plays an irreplaceable role in agricultural engineering, biomedicine and other fields. The action of PEF is primarily concentrated on the cell membrane and nuclear membrane. It generates potential differences between the insides and outsides of biofilms, forming transmembrane voltage. When the transmembrane voltage exceeds a certain threshold, it causes physiological changes in the cells and eventually leads to irreversible electroporation of the biofilm to induce apoptosis or cell death [2]. Its hyphae are tubular filaments which can elongate and branch. Many branched hyphae interweave with one further to form mycelium, and the mycelium may contain multiple nuclei
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